Quenching autofluorescence

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From:Corazon Bucana <bucana@audumla.mdacc.tmc.edu>
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I am working with paraffin embedded samples, mainly archival specimens, and
I am getting very intense autofluorescence of red cells and connective
tissueswhen using the FITC or Rhodamine filter sets.  Has anyone tried
quenching this autofluorescence?  I would appreciate any comments.

Corazon Bucana
Corazon D. Bucana, Ph.D.
Department of Cancer Biology
U.T. M.D. Anderson Cancer Center
1515 Holcombe Blvd.  Box 173
Houston, Texas 77030
Phone: (713) 792-8106
FAX: (713) 792-8747
FAX: (713) 792-8747

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