Quenching autofluorescence
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From: | Corazon Bucana <bucana@audumla.mdacc.tmc.edu> |
To: | HistoNet@Pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
I am working with paraffin embedded samples, mainly archival specimens, and
I am getting very intense autofluorescence of red cells and connective
tissueswhen using the FITC or Rhodamine filter sets. Has anyone tried
quenching this autofluorescence? I would appreciate any comments.
Corazon Bucana
*******************************************************
Corazon D. Bucana, Ph.D.
Department of Cancer Biology
U.T. M.D. Anderson Cancer Center
1515 Holcombe Blvd. Box 173
Houston, Texas 77030
Phone: (713) 792-8106
FAX: (713) 792-8747
Email:bucana@audumla.mdacc.tmc.edu
FAX: (713) 792-8747
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