Quenching autofluorescence

<< Previous Message | Next Message >>
From:Corazon Bucana <bucana@audumla.mdacc.tmc.edu>
To:HistoNet@Pathology.swmed.edu
Reply-To:
Content-Type:text/plain; charset="us-ascii"

I am working with paraffin embedded samples, mainly archival specimens, and
I am getting very intense autofluorescence of red cells and connective
tissueswhen using the FITC or Rhodamine filter sets.  Has anyone tried
quenching this autofluorescence?  I would appreciate any comments.

Corazon Bucana
*******************************************************
Corazon D. Bucana, Ph.D.
Department of Cancer Biology
U.T. M.D. Anderson Cancer Center
1515 Holcombe Blvd.  Box 173
Houston, Texas 77030
Phone: (713) 792-8106
FAX: (713) 792-8747
Email:bucana@audumla.mdacc.tmc.edu
FAX: (713) 792-8747




<< Previous Message | Next Message >>