calling IEM experts

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From:Jeff Crews <>
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        It looks like I'm going to have to do some immunoelectronmicroscopy 
     soon. I'll probably be looking for elastin in the dermis of a skin 
     construct. I've done plenty of ultrastructural work on collagen and 
     other skin components, but this will be my first IEM.
        So, does anyone have any recommendations for IEM in the skin? Are 
     you doing colloidal gold techniques, or something else? And most 
     importantly, how are you fixing the tissues, and are you labeling 
     before or after processing and embedding?
        I'd appreciate hearing from anyone doing similar techniques, or 
     even just some good references. Thanks a lot.
     Jeffrey Crews, HTL (ASCP)
     Organogenesis, Inc.

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