Formalin fixed tissues
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From: | MICHELLE LOWE <lowzey@yahoo.com> |
To: | Histologists <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset=us-ascii |
I was told by a co-worker: the longer tissue is kept
in formalin, the more it losses its staining
properties. We were discussing keeping wet tissue vs.
blocks for back up special stain/immunohistochemistry
controls.
Does it really matter how long the tissue is in
formalin?
What are the effects of tissue kept too long in
formalin?
It is ideal to have 5-10 year old blocks to use as
controls?
I know that this question seems a little generic, but
I'm still a rookie!! :)
Michelle Lowe
Sup. Anatomic Pathology/Histology
Valley Children's Hospital
Madera, CA
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