CD34 followup
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From: | "Connolly, Brett" <brett_connolly@merck.com> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Thanks for all of your responses to my CD34 question. As it turns out I
really need anti-mouse CD34 for paraffin sections.
Brett
Here is a sampling of the responses for human CD34.
Yes, Brett; we use Immunotech's CD34 at 1:200 with a 20" HIER pretreatment.
Hello Brett,
I use Becton-Dickinson's CD34 cat.# 347660 on formalin fixed paraffin
embeded tissue at a 1:60 dilution on the Dako autostainer. I pretreat
with citra buffer for 15 minutes. Nice results. Good Luck.
Zymed carries cd34,very good, so does signet .
Hello Brett,
InnoGenex carries an anti-human CD34 antibody that is validated on
formalin-fixed, paraffin embedded tissue(Clone:QBEnd/10, Catalog No.
AM-1235-01).
The data sheet can be accessed online:
http://www.innogenex.com
Hi Brett,
We use Becton-Dickenson's (#347660) clone HPCA-1 on our NexES. We run it at
a 1
:5 dilution for 24 min. and no pretreatment. Works fine.
Try NCL-END from Nova Castra.
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