Hi Dana
Two clones used to be available from the Biological Materials Distribution
Center at the Washington Research Foundation (sorry, I have no current web
address): clone CF-2, which is genus-specific, and clone RR402, which is
Chlamydia pneumoniae (TWAR)-specific. We used them at dilutions of
1:200-1:1000 (CF-2; approx. 1-5 ug Ig/ml) and 1:50-1:250 (RR402; approx.
4-20 ug/ml) after antigen retrieval in 10 mM citrate buffer, pH 6.0,
pressure cooker, StrABC-visualization. Staining is one thing, interpretation
is the other ... Reactivity in mast cells is sometimes observed, as well as
non specific staining of connective tissue. Good luck!
Andi Kappeler
Institute of Pathology, University of Bern
Switzerland