Re: Citrate buffer
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet@histosearch.com |
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> used in antigen retrieval. I currently use a combination of citric acid
> and sodium citrate and recall there were some variations. Your input
That's exactly what a citrate buffer is! Adjust the pH
by adding more acid to lower it, or more trisodium citrate
to get a higher pH. This system is effective in the range
3.0 to 6.2. Anything more alkaline is not a buffer, even if
it successfully retrieves antigens. Citrate ions may, like
EDTA ions, contribute to antigen retrieval by chelating calcium
as well as by providing a favourable pH for the reversal of some
of the reactions of fixation by formaldehyde. For an interesting
and brief exposition of this theory, read the abstract by Jasani
et al. in Histochemical Journal vol 29 (issue 6) p. 433.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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