Dear Netters,

Happy New Year!  We are serial-sectioning medaka fish for BrdU
staining.  Generally, in order to section several sections at a time, we
rub the block with ammonia water.  However, we are not sure whether or
not the ammonia will have a negative affect on the BrdU staining.  Does
anyone have any insight into these matters?  Thank you in advance.

Sincerely,

Beth Poole
Histo-Scientific Research Labs.
107 Killmon Road
Basye, VA  22810
(540)856-2222
fax: (540)856-2227
histosci@shentel.net