Hi,
    We have noticed similar antigenic changes. Our MEL-5 tends to be
stronger in recently cut controls than in those that have sat for a while.
ok back to my cartoons :-)
Amos Brooks

Colleen Forster wrote:

> I agree you cannot stain ER/PR, D1 cyclins, BrDu's on cut slides that
> have sat for long periods of time and expect good results. They will
> need to be protected. A majority of antigens will be forgiving however,
> I would not put nuclear antigens into that category.
>
> The paraffin wax technique sounded interesting and I would wonder how
> that works on these difficult antigens ??
>
> Colleen Forster
> U of MN, Dept. of Neurology
> 612 626-2477