Re: Cyclin D1 and CD5 immunohistochemistry
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From: | Phyllis Davie <pdavie@phenopath.com> (by way of histonet) |
To: | histonet@histosearch.com |
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Content-Type: | text/plain; charset="us-ascii" |
Melanie,
We have had very good luck with CD5. I hope we are both talking about
human tissue, by the by. CD5 clone 4C7, from Novocastra (Australian
contact info: PO Box 193, Rockdale, NSW 2216, Tel: 61-02-9597-3688 Fax:
61-02-9597-2232, e-mail: info@austlabservices.com.au)
We pretreat in citrate buffer in a microwave pressure cooker for 10
minutes at pressure, and run it at 1:25 using an ABC detection system and
DAB chromogen.
Cyclin D1 has been trickier, and doesn't look as nice, but this is our
best so far:
cyclinD1, clone DR1BC2, Biocare Medical (I don't have Australian info,
but USA info is Phone: 800-799-9499, 888-531-8287 Fax: 925-944-7888)
We pretreat in a vegetable steamer for 40 minutes in (preheated) pH 10
Tris buffer. We run it at 1:25 using an ABC detection system and DAB
chromogen.
Good Luck,
Phyllis Davie
PhenoPath Laboratories
Seattle, WA 98121
pdavie@phenopath.com
>Dear Histonetters,
>
>I am keen to hear of success stories for immunohistochemistry of cyclin D1
>and CD5 on formalin fixed sections. Anyone willing to share protocols,
>choice of primary antibodies? Thanks in anticipation.
>
>Melanie.
>
>
>Melanie Trivett, PhD
>Department of Pathology
>Peter MacCallum Cancer Institute,
>East Melbourne, Victoria, Australia.
>
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