overnight incubation.

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From:Masayuki Miyagishima <mmiyagis+@pitt.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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I am doing NF-kB staining, but can not stain well inside the cell
(especially inside nuclei).  We use frozen section and now started to
try Saponin and proteinase K before the staining.  Some people in other
lab suggeted me to do overnight incubation for normal goat serum
blocking, 1st antibody and secondary antibody(anti-rabbit, fitC
conjugated).  I have never done overnight for blocking or secondary.
Doesn't it do harm for fluorescence?

Thank you in advance.

Masayuki Miyagishima, MD
University of Pittsburgh,
Department of Surgery

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