Re: DiI compatibility

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From:Mick Rentsch <> (by way of histonet)
To:histonet <>
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Dear Karen,
you could try either Oil Red O or Sudan Black on frozen sections, using
isopropanol as solvent,  or perhaps higher MW alcohol. Check the solubility
of Dil first just to make sure you don't elute it.
Regards Mike Rentsch (Downunder)
-----Original Message-----
From: Karen D. Larison <>
To: <>
Date: Saturday, 19 December 1998 6:20
Subject: DiI compatibility

>Hello Histonetters,
>Many of you recommended that my graduate student use luxol fast blue
methods to
>stain myelin.  So this is what he plans to do.  However, the graduate
>used DiI to mark the electrode track in this experiment, and it is soluble
>the alcolhols used in the luxol blue methods.  Currently, he plans to stain
>every other section with the luxol blue protocol so that he still has a
>of the electrode track.  Unfortunately, this is an animal that has been
>repeatedly poked, so he can't simply reply on tissue damage to identify the
>electrode track.  Are there any alternate methods for identifying the
>For instance, are any of the myelin-specific antibodies reliable for this
>application?  He's looking at monkey.
>Karen D. Larison

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