Re: Myeloperoxidase Staining

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From:Katie Bennett <> (by way of histonet)
To:histonet <>
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To: Clif Chapman, Cathy Fragiskatos, and any other interested parties.

What kind of antibody do you use against myeloperoxidase?  Does it stain

I am trying the myeloperoxidase assay from the AFIP manual with little
success.  Here's what I'm trying to do:

I am trying to do a stain that will allow easy detection for
quantitation of all the neutrophils in formalin-fixed, paraffin-embedded
tissues so I can tell them apart from eosinophils and not rely solely on
the morphology of the nucleus.  We have found that a May-Grunwald stain
is great for the eos.  We probably could count the neutrophils in these
May-Grunwald stained sections, but I would like to find something that
makes those neutrophils really stand out.  I am not planning on doing
both stains on one slide, but on serial sections.  However, a good  IHC
stain for neutrophils with a May-Grunwald counterstain would be awesome!

Thanks in advance for any info you might have!

Catherine "Katie" Bresee Bennett
218G Food Safety Toxicology Building
Department of Pathology
Michigan State University
East Lansing, MI 48824

ph:  (517) 432-4940
fx:   (517) 353-9902

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