Re: HIER in 0.4M EDTA?

<< Previous Message | Next Message >>
From:Patsy Ruegg <> (by way of histonet)
To:histonet <>
Content-Type:text/plain; charset="us-ascii"

You are right, it should be a 1mM solution of EDTA Ph 8.0 for HIER.  I
was confusing my digestion with EDTA procedure for the HIER procedure.
I use the EDTA for HIER in a pressure cooker in a 750 watt microwave on
high for 15 min.  We are starting to switch a lot of our protocols which
used citrate buffer Ph 6.0 to the EDTA retreival solution.

<< Previous Message | Next Message >>