Re: HIER in 0.4M EDTA?
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| From: | Patsy Ruegg <rueggp@earthlink.net> (by way of histonet) |
| To: | histonet <histonet@magicnet.net> |
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| Content-Type: | text/plain; charset="us-ascii" |
Rod,
You are right, it should be a 1mM solution of EDTA Ph 8.0 for HIER. I
was confusing my digestion with EDTA procedure for the HIER procedure.
I use the EDTA for HIER in a pressure cooker in a 750 watt microwave on
high for 15 min. We are starting to switch a lot of our protocols which
used citrate buffer Ph 6.0 to the EDTA retreival solution.
Patsy
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