Re: Crystal mount -Reply

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From:Katri Tuomala <> (by way of histonet)
To:histonet <>
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Karen K. Dulany wrote:
> I have used the Crystal mount, and then I applied the coverslip to
> it, but have never heard of putting it on the slide, letting it dry,
> and then coverslipping it the next day with xylene and permount.
> Perhaps someone else has tried this.
> Karen Dulany
I used Crystal Mount for several years, when using AEC as chromogen. I
found that it preserved the staining well except maybe turned AEC a bit
brownish in time. I did also on occasion applied a coverslip on it with
Permount to mainly achieve a better optical resolution for
microphotography. It would not be cost effective to do on all slides.
Crystal Mount itself is expensive, which was one of the reasons we
eventually switched over to DAB. Katri:-)

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