Re: [Histonet] nuclei extraction from paraffin slides

From:Rene J Buesa

Gudrun:
  We used to so something similar (for flow cytometry) on whole prostate large specimens.
  First we selected (from the stained slide) the area we wanted. From that are we took a "core" biosy, reembedded it and section at 200 Ám steps.
  Those thick slices were treated with xylene (and hydrated to saline solution) to eliminate the paraffin. Later they were disintegrated by shaking them in saline solution until the components were dislogged.
  That was the material used for flow cytometry and the results were good.
  Hope this will help you!
  RenÚ J.

Gudrun Lang  wrote:
  Hi,

I am looking for a good protocol for extracting nuclei from paraffin slides.
The nuclei suspension should serve for FISH-testing on lymphoma-material.

Perhaps someone could share his/her protocol with me.



Thanks in advance



Gudrun Lang

Histologie

Linz, Austria





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