RE: [Histonet] FITC C4d

From:"Terri Braud"

Ditto on Quidel's C4d performance, its great! It is listed as a RUO which is no biggie, as long as you've done your homework, documentation and have an ASR disclaimer on any patient report.  It still takes interpretation and correlation with clinican results.  We used it when looking at frozen section renal allografts with cell mediated rejection at 1:20 and the goat anti mouse FITC at 1:10 
Terri Braud

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Traci
Sachs
Sent: Wednesday, December 06, 2006 2:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] FITC C4d


Donna,

=0AQuidel carries a C4d anibody ( not indirect labeled ) for FITC.  The 
=0Asecondary is a Goat anti-mouse FITC.  The item number was A213 and this 
=0Aworked well at a dilution of 1:40 made in normal goat serum on frozen 
=0Atissue.  The Quidel antibody is an ASR.


>From: "Richard Cartun" 
>To: "Donna Lawson" , 
>,"Mark Tarango" 
>Subject: RE: [Histonet] FITC C4d
>Date: Wed, 06 Dec 2006 09:45:27 -0500
>
>I thought the disclaimer was for analyte-specific reagents (ASRs) only?
>
>Richard
>
>Richard W. Cartun, Ph.D.
>Director, Immunopathology & Histology
>Assistant Director, Anatomic Pathology
>Hartford Hospital
>80 Seymour Street
>Hartford, CT  06102
>(860) 545-1596
>(860) 545-0174 Fax
=0A>
> >>> "Tarango, Mark"  12/05/06 5:55 PM >>>
>I've tried and didn't find one.  It's all about the good faith effort,
>if you ask me. . .
>
>You have that FDA disclaimer on the reports afterall, right?
>
>
>
>Mark Adam Tarango HT(ASCP)
>
>Histology/Immunohistochemistry Supervisor
>
>Nevada Cancer Institute
>
>One Breakthrough Way
>
>Las Vegas, NV  89135
>
>mtarango@nvcancer.org
>
>Direct Line (702) 822-5112
>
>Fax (702) 939-7663
>
>
>
>
>-----Original Message-----
>From: histonet-bounces@lists.utsouthwestern.edu
>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna
>Lawson
>Sent: Tuesday, December 05, 2006 12:03 PM
=0A>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] FITC C4d
>
>HI,
>
>Is anyone out there using an indirect FITC labeled C4d antibody that
>is
>NOT an RUO? I can only find 2 and they both say for RUO not for
>Clinical
>Diagnostics.
>
=0A>Any Help will be greatly appreciated,
>
>Donna Lawson H.T. (ASCP) QIHC
>Histology Supervisor
>University of Kansas Hospital
=0A>913-588-1134
>
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>
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