[Histonet] mouse heart valves

From:"Danielle Crippen"

Dear All,

I need to process immersion fixed (NBF) mouse heart for optimum morphological detail of the valves.  Can anyone advise on the best approach??  Is FFPE or cryoprotection/freezing more recommended?  Also, is 24h immersion fixation sufficient or does it need to go longer/shorter??  Any advice will be greatly appreciated!!

Many thanks!


Danielle Crippen

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