RE: [Histonet] mouse heart valves

From:"Liz Chlipala"


Are you talking about mouse aortic root sections from animal models of
atherosclerosis?  If that's the case then depending upon how you are going
to analyze the specimens you can process to paraffin or cut frozen sections.
We snap freeze the heart and then cut frozen sections, since we want to be
able to stain for fat with Oil Red O, but there are publications that
perform paraffin sections but their image analysis of the root sections is
different than the Oil Red O frozen specimens.  We do this quite a bit so
you can contact me and I'll go over the instructions on how to section to
the correct area and how many sections we take and how we handle them, etc,
it can be quite technical and labor intensive initially when working with
these samples, but once you get the hang of it, its much better.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
[] On Behalf Of Danielle
Sent: Friday, December 08, 2006 12:13 PM
Subject: [Histonet] mouse heart valves

Dear All,

I need to process immersion fixed (NBF) mouse heart for optimum
morphological detail of the valves.  Can anyone advise on the best
approach??  Is FFPE or cryoprotection/freezing more recommended?  Also, is
24h immersion fixation sufficient or does it need to go longer/shorter??
Any advice will be greatly appreciated!!

Many thanks!


Danielle Crippen

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