Re: [Histonet] negative controls with IHC
Lisa,
Your best bet is to contact CAP directly with your question(s). They
have an e-mail address that I have used several times. They answer
promptly and precisely.
The address is:
www.accred@cap.org
Good Luck,
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
>>> lwuerges 12/12/2005 7:25:00 PM >>>
I have a question regarding negative controls for a manual
immunoperoxidase procedure. Do you use a neg control for each antibody
used with each block? or one neg control per block regardless of how
many antibodies for that block. The latter is what I was told, but the
first seems more likely on account of each antibody may be treated
differently with some needing antigen retrieval--obviously the contol
should receive identical treatment. What is the usual neg control
protocol?
Thank you.
Lisa Wuerges
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