Re: [Histonet] fixation in 70% alcohol
70% ethanol is not considered a fixative. Ethanol fixes by dehydration, so
at a minimum you should be using 95% ethanol, and preferably absolute. 70%
can be used as a preservative after fixation. You have unfixed tissue if
they are put into it immediately after harvest. Since it is not a fixative,
the length of time is not really a factor. What is most likely happening is
that you then dehydrate with higher concentrations of ethanol and the tissue
is fixed at that time. Ethanol alone is considered to be a very poor
fixative, although it may be necessary depending on your application. I
suggest you either use a proper fixative such as a formalin variant,
preferably overnight, or put your tissue directly into absolute ethanol for
a few hours.
----- Original Message -----
From: "Fawn Jones"
Sent: Thursday, December 08, 2005 7:03 AM
Subject: [Histonet] fixation in 70% alcohol
>I just started working in a new lab and they use 70% Ethanol for fixation,
>however I do not know how long to keep the tissues in 70% to ensure
>adequate fixation. I am working with animal bones (mice, rats, sheep).
>Could anyone give me suggestions on fixation times?
> Thank you
> Fawn Jones
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