RE: [Histonet] coverslipping bubbles

From:"Patsy Ruegg"

Good idea, I will have to try that with something like tween.

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
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-----Original Message-----
[] On Behalf Of Favara,
Cynthia (NIH/NIAID) [E]
Sent: Thursday, December 08, 2005 9:04 AM
To: Histonet
Subject: [Histonet] coverslipping bubbles

I was wondering if anyone has tried a wetting agent in the distilled water
prior to coverslipping with aqueous mounting media? If so what did you use
and at what concentration. I just thought it might help with my constant
bubble problem on immunos.





Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

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