[Histonet] (no subject)

From:"Patterson, Pat"

Hello to all HistoNet folks.  


Our lab is having inconsistent occurrences of "crispy" biopsies - especially
Livers. They feel crispy during embedding and sectioning and the sections
appear to have cracking - separating the tissue in unnatural directions.


These are fixed in Carson Millonig formalin and then processed with 10% NBF,
70% alc, 95% alc, Abs alc, xylene to paraffin - total process about 2.5
hours.  They are held in the 10% NBF at the beginning of the processing
schedule and there is no heat on the retort until the paraffin steps.


The biopsies are placed in "micro-cassettes" with no wrapping (these
cassettes are not the micro-screen cassettes ones with metal like


We'd appreciate any ideas you might have - especially since our liver
transplant service is increasing in volume as I type!




Pat Patterson

Manager, AP

Methodist Dallas Medical Center

(214) 947-3538




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