RE: [Histonet] (no subject)
It sounds like processing issue being carried out too fast.
From: Patterson, Pat [mailto:PatPatterson@mhd.com]
Sent: 12 December 2005 14:48
Subject: [Histonet] (no subject)
Hello to all HistoNet folks.
Our lab is having inconsistent occurrences of "crispy" biopsies - especially
Livers. They feel crispy during embedding and sectioning and the sections
appear to have cracking - separating the tissue in unnatural directions.
These are fixed in Carson Millonig formalin and then processed with 10% NBF,
70% alc, 95% alc, Abs alc, xylene to paraffin - total process about 2.5
hours. They are held in the 10% NBF at the beginning of the processing
schedule and there is no heat on the retort until the paraffin steps.
The biopsies are placed in "micro-cassettes" with no wrapping (these
cassettes are not the micro-screen cassettes ones with metal like
We'd appreciate any ideas you might have - especially since our liver
transplant service is increasing in volume as I type!
Methodist Dallas Medical Center
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