[Histonet] MMP-1 in Bouin's fixed tissue

From:"Mark Elliott"

Has anyone had any experience staining for MMP-1 in Bouin's fixed human
tissues?  One of the Hospital's we collaborate with fixes their lungs in
Bouin's.  We have tried staining with no antigen retrieval and
with/without overnight incubation in primary.  Have also tried antigen
retrieval using citra pH 6 in 95 C water bath and in autoclave with no
luck.  We are using MAB 1346 from Chemicon followed by APAAP technique. 
Are going to try high pH antigen retrieval and possibly trypsin but
would appreciate any other suggestions.

Mark Elliott
Vancouver BC

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