[Histonet] Steiners problems-milky staining on pt tissue

From:"Stoll, Kathy"

Our lab has been having problems with our Steiners stain for the past
several months.  Our control slide usually turns out good, but the patient
slide has milky grey coloring under the scope.  Grossly the patient tissue
has a gold coating.  Occasionally both the patient and control slides have
background staining, but not always. 
We use the following 10% NBF, control slides cut 2-3 months ago.
We have tried the following...different batch of slides, making new
solutions (we mix our own), acid cleaning the patient slide, glass coplin
jars instead of plastic, different temperatures primarily for reducing
solution, bottled water instead of tap distilled.
This problem is recent and one that we had never had before.  
Thank you in advance for your help in solving our mystery.

Kathy Stoll, HT
St. Joseph's Community Hospital
West Bend WI

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