RE: [Histonet] Steiners problems-milky staining on pt tissue

From:"Weems, Joyce"

Have you had any B5 fixative anywhere - tissue processed in the sameprocessor?-----Original Message-----From:[]On Behalf Of Stoll,KathySent: Thursday, January 08, 2004 2:58 PMTo: ''Subject: [Histonet] Steiners problems-milky staining on pt tissueOur lab has been having problems with our Steiners stain for the pastseveral months.  Our control slide usually turns out good, but the patientslide has milky grey coloring under the scope.  Grossly the patient tissuehas a gold coating.  Occasionally both the patient and control slides havebackground staining, but not always. We use the following 10% NBF, control slides cut 2-3 months ago.We have tried the following...different batch of slides, making newsolutions (we mix our own), acid cleaning the patient slide, glass coplinjars instead of plastic, different temperatures primarily for reducingsolution, bottled water instead of tap distilled.This problem is recent and one that we had never had before.  Thank you in advance for your help in solving our mystery.Kathy Stoll, HTSt. Joseph's Community HospitalWest Bend WI**********************************************************************This email and any files transmitted with it are confidential andintended solely for the use of the individual or entity to whom theyare addressed. If you have received this email in error please notifythe system manager.This footnote also confirms that this email message has been swept byMIMEsweeper for the presence of computer**********************************************************************_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.edu Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer.Thank you. Saint Josephs Health System, Inc.

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