[Histonet] RE: [IHCRG] IHC on cells on coverslips

From:Tony Henwood

Depending on the antibody. Most work after ethanol fixation, though some
require air-drying-cold acetone treatment.
You could attach a small paper clip to a corner of the coverslip to aid

Suggest fixation in 95% ethanol, 20min. Block endogenous enzyme (if
required), rinse in buffer.
You might consider marking the back of the coverslip with a marking pen at
this stage (the ink will dissolve in the dehydrating solutions). This will
aid in orientation.
Proceed with immunohistochemistry.
Remember to not let the coverslip dry out.
Mount Coverslip with a slide.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318


-----Original Message-----
From: Patsy Ruegg [mailto:pruegg@colobio.com]
Sent: Wednesday, 10 December 2003 9:12 AM
To: Histonet@Pathology. Swmed. Edu
Cc: Ihcrg@Yahoogroups. Com
Subject: [IHCRG] IHC on cells on coverslips

Please advise how to manage IHC staining for cells grown on coverslips.

------------------------ Yahoo! Groups Sponsor ---------------------~-->
Buy Ink Cartridges or Refill Kits for your HP, Epson, Canon or Lexmark
Printer at MyInks.com. Free s/h on orders $50 or more to the US & Canada.

To unsubscribe from this group, send an email to:


Your use of Yahoo! Groups is subject to http://docs.yahoo.com/info/terms/ 

This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please 
delete it and notify the sender.

Views expressed in this message and any attachments are those 
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been 
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any 
consequential damage resulting from email containing computer 

Histonet mailing list

<< Previous Message | Next Message >>