RE: [Histonet] Steiner and Steiner method/H. pylori

From:Tony Henwood

For HP, the Warthin Starry modifications seem to be the best (?gold
Unfortunately many of us find the stain complex and long. Not a good routine
stain for most labs.
You can replace the uranyl nitrate with Zinc Formalin, Lead nitrate and
ferric alum

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: Derek and/or Lynda Leopold []
Sent: Wednesday, 10 December 2003 8:30 AM
Subject: [Histonet] Steiner and Steiner method/H. pylori

Hi list,
Would anyone care to comment on the Steiner and Steiner (microwave) 
method for spirochetes?  I susupect my lab's pathologists are interested 
in it for viewing H. pylori, yet in all the "HP" talk on this list I 
have yet to hear it mentioned.  How does it compare from a tech safety 
standpoint?  As a brand-new tech, I still do crazy things like read the 
safety warnings on ingredients, and that whole uranyl nitrate 
radioactive thing is sort of bothering me.  We use no protective 
equipment when doing this stain (which the doctors still call "Warthin 
Starry", incidentally, but hey---they're making the big bucks....)
I'll take anyone's 2 cents...
Lynda Leopold

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