RE: Fixing a Frozen Section Slide.

From:Cheryl Powell

What about for IHC?  I've read acetone or methanol but haven't any experience.  How does it work for ya'll out there?

Cheryl Ann Powell B.S., HTL(ASCP)
Botsford General Hospital
Farmington Hills, MI USA
>From: Tony Henwood
>To: 'Banjo Adesuyi' , "''"
>Subject: RE: Fixing a Frozen Section Slide.
>Date: Thu, 19 Dec 2002 08:43:53 +1100
>Depends on what you are staining for eg:
>For H&E, Perl's PAS ... I would recommend Ethanol
>Gomori's Trichrome for Muscle biopsies - air dried
>For IPs either air dried or ethanol depending on the antibody
>Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
>Laboratory Manager
>The Children's Hospital at Westmead,
>Locked Bag 4001, Westmead, 2145, AUSTRALIA.
>Tel: (02) 9845 3306
>Fax: (02) 9845 3318
>-----Original Message-----
>From: Banjo Adesuyi []
>Sent: Monday, 16 December 2002 3:57
>Subject: Fixing a Frozen Section Slide.
> Please I am wondering If you guys could advice me on the best way to fix
>a frozen section slide before staining.
> You people are doing a wonderful job. You are the best.
> Ade.
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