Immunohistochemistry on sawn section
Is anyone of you familiar with immunohistochemistry on sawn PMMA section? I've already tried this a few times, but when I try to remove the PMMA-resin the slices come loose. Normally I use a UV-curing glue for fixing the slices onto the glass, but then I can only perform a surfacestain on the slices (after a brief resin-removal).
Also I've done some testing with silane-treated glassware. This helps a jus a bit. Recently I had one succesfull effort (just one) with silane-treated slide together with Loctite 401 superglue. The result was a fine HE-stained slice, but when I tried to do immunology on another slide the result where as usual (wich means bad).
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