In my experience, it depends. I have had very good results with GFAP immunostaining after several months of storage. On the other hand, immunostaining for tyrosine hydroxylase was noticably degraded in finer nerve fibers but cell bodies were OK.
    Optimal storage conditions may vary depending on what you are looking for.

Geoff

d.segers@planet.nl wrote:

> Dear Histonetters,
>
> Just a simple question. How long can frozen unfixed sections be stored in a -60 C freezer. Sections are mounted on superfrost plus slides. Does anyone have experience with storage of sections, or does anyone have some advice on how to optimally store our material.
>
> Dolf Segers
> Dept. Experimental Cardiology, Thoraxcenter
> Erasmus MC, Rotterdam

--
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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