Just to clarify my original question, the pathologist wants use the tetracycline labeling to study osteoid seams of bone biopsies, not bone marrow biopsies as the subject eludes to. Thanks again.

Charnell E. Jones, BS, HTL(ASCP)
CompuNet Clinical Laboratories

> -----Original Message-----
> From:	Jones, Charnell E 
> Sent:	Monday, December 02, 2002 3:07 PM
> To:	'histonet@pathology.swmed.edu'
> Subject:	Tetracycline Labeling of Bone Marrow Biopsies
> 
> Hi All,
> 
> I have a pathologist that is interested in labeling bone marrow biopsies with the antibiotic tetracycline and then using fluorescence to visualize. Is anyone familiar with this technique? I found a reference for this technique in Sheehan for methacrylate embedded sections, but I wanted to know if this could be done on paraffin processed tissue? If you have any other references or procedures for this technique, I would appreciate it. Thanks in advance.
> 
> Charnell E. Jones, BS, HTL(ASCP)
> CompuNet Clinical Laboratories
> Dayton, OH