Tetracycline Labeling of Bone Marrrow Biopsies

From:"Jones, Charnell E"

Hi All,

I have a pathologist that is interested in labeling bone marrow biopsies with the antibiotic tetracycline and then using fluorescence to visualize. Is anyone familiar with this technique? I found a reference for this technique in Sheehan for methacrylate embedded sections, but I wanted to know if this could be done on paraffin processed tissue? If you have any other references or procedures for this technique, I would appreciate it. Thanks in advance.

Charnell E. Jones, BS, HTL(ASCP)
CompuNet Clinical Laboratories
Dayton, OH 

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