RE: Cracks in Paraffin Wax Blocks
If you have a similar pattern of cracks on each block, you might want to
look at how they are being handled. I have noticed that blocks I do
research on from overseas have the same cracks in 75% of them from rough
handling. If the cracks are very random, I would look and either your
paraffin or your humidity levels where they are stored.
From: ncragg [mailto:firstname.lastname@example.org]
Sent: Wednesday, December 04, 2002 10:53 AM
To: 'HistoNet Server'
Subject: Cracks in Paraffin Wax Blocks
Please could anybody tell me why I am getting cracks in my paraffin wax
blocks? I noticed someone had asked this question a couple of years ago
but there was no reply posted. I can still cut sections from these blocks
and they look fine under the microscope. Not all the blocks in a batch are
cracked, some are nice and I've even had batches where 90% of the blocks
are good and only a few have cracks.
We use Surgipath Formula R paraffin wax and I have spoken to the rep there
and he suggested a couple of things, but none have made any difference; he
has also checked the batch of wax we were using and there are no reported
problems; besides we are now onto another batch and the same thing happens.
I have wondered whether it is my embedding technique, but have tried many
different ways, e.g. different amounts of wax in the base mould before
orientation, different lengths of time on the refrigeration spot before
topping up and different methods of cooling, ie. using the coldplate and
-20 freezer. I am also careful not to move the tissue once the wax starts
to solidify. The wax is melted at 60 degrees celsius in the processor and
the embedding centre is also set at 60.
I would be very grateful if anyone has anyone got any suggestions?
Thanking you in advance.
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