We have had success in using Vectabond (Vector Labs) coated slides to
prevent tissue lifting. It works well. 

Parthak Prodhan
MassGeneral Hospital
Boston, MA 02114
617-724-2894
> 	----------
> 	From:  Marcus Andrews[SMTP:m.andrews@utoronto.ca]
> 	Sent:  Wednesday, December 04, 2002 4:30 PM
> 	To:  Histonet@pathology.swmed.edu
> 	Subject:  poly-lysine coated slides
> 
> 	Dear all:
> 
> 	Is anybody collecting frozen tissue specimens on poly-lys coated
> glass 
> 	slides?  In order to save cost, we manually coat glass slides with 
> 	poly-L-Lysine (from Sigma), and keep them refrigerated at +4oC for a
> 
> 	max time of 2-3 weeks before deeming them unusable (presumably due
> to 
> 	poly-Lys breakdown).  As a large part of our target tissue is
> neural, 
> 	we prefer to use this method of coating slides.
> 
> 	Does anybody have any clues if coated slides can last for longer 
> 	periods without losing their Lys-charge?  I'm finding that too many 
> 	slides are being wasted due to the time barrier and am not
> particularly 
> 	sure it's reasonable (however, don't want my analysis to suffer due
> to 
> 	tissue/slide problems!).
> 
> 
> 	.:---------------------------:.
> 	   Marcus Andrews, PhD.
> 	   Department of Physiology,
> 	   Faculty of Medicine,
> 	   University of Toronto,
> 	   1 King's College Circle,
> 	   Toronto, Ontario,
> 	   M5S 1A8
> 	':---------------------------:'
> 
> 	
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