RE: poly-lysine coated slides
|From:||"Prodhan, Parthak,M.D." |
We have had success in using Vectabond (Vector Labs) coated slides to
prevent tissue lifting. It works well.
Boston, MA 02114
> From: Marcus Andrews[SMTP:email@example.com]
> Sent: Wednesday, December 04, 2002 4:30 PM
> To: Histonet@pathology.swmed.edu
> Subject: poly-lysine coated slides
> Dear all:
> Is anybody collecting frozen tissue specimens on poly-lys coated
> slides? In order to save cost, we manually coat glass slides with
> poly-L-Lysine (from Sigma), and keep them refrigerated at +4oC for a
> max time of 2-3 weeks before deeming them unusable (presumably due
> poly-Lys breakdown). As a large part of our target tissue is
> we prefer to use this method of coating slides.
> Does anybody have any clues if coated slides can last for longer
> periods without losing their Lys-charge? I'm finding that too many
> slides are being wasted due to the time barrier and am not
> sure it's reasonable (however, don't want my analysis to suffer due
> tissue/slide problems!).
> Marcus Andrews, PhD.
> Department of Physiology,
> Faculty of Medicine,
> University of Toronto,
> 1 King's College Circle,
> Toronto, Ontario,
> M5S 1A8
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