I'm trying out, what works better for ISH (fixing the fresh tissue) and I do
think both work equally well. This makes me wonder if the same logic could
be applied to the para step during ISH? Has anyone got experience? Why
should I have to use para there? My probes are oligos (20mer), by the way.
This could make some difference. They are more stable - easier to handle...
If I don't have to use para, I won't, what a waste of time preparing it. But
perhaps I'm wrong somewhere?

Margarete Vermehren
LMU M#252#nchen / Tieranatomie II
Tel:  +49 89 21805857
Fax: +49 89 21802569
m.vermehren@anat.vetmed.uni-muenchen.de