|From:||Rena Fail |
We use Kappa and Lambda from DAKO on the Dako autostainer with Envision +.
Kappa with proteinase K,
Lambda 1:1500 with no antigen retrieval
I am at home and do not recall the dilution for Kappa. The real key to getting consistent staining with no background for these abs particularly Kappa was the use of a protein block.
From: Cheryl Powell [mailto:email@example.com]
Subject: Kappa/Lambda IHC
Hi, Botsford recently started up an immuno program and
we're constantly adding antibodies. Kappa/Lamda has been a real
pain. We've tried several companies and so many varations it makes my
head spin- but nothing is consistant. What companies, dilutions, and
antigen retrievals are any of you using? Do you have alot of background
or nonspecific staining?
Cheryl Ann Powell B.S., HTL(ASCP)
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