| From: | Rena Fail |
Cheryl,
We use Kappa and Lambda from
DAKO on the Dako autostainer with Envision +.
Kappa with proteinase K,
Lambda 1:1500 with no antigen retrieval
I am at home and do not recall the dilution
for Kappa. The real key to getting consistent staining with no background for
these abs particularly Kappa was the use of a protein block.
Rena Fail
-----Original Message-----
From: Cheryl Powell
[mailto:cherylhisto@hotmail.com]
Sent:
To: histonet@pathology.swmed.edu
Subject: Kappa/Lambda IHC
Hi, Botsford recently started up an immuno program and
we're constantly adding antibodies. Kappa/Lamda has been a real
pain. We've tried several companies and so many varations it makes my
head spin- but nothing is consistant. What companies, dilutions, and
antigen retrievals are any of you using? Do you have alot of background
or nonspecific staining?
Cheryl Ann Powell B.S.,
HTL(ASCP)
the new MSN 8
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