Kappa/Lambda IHC

From:Rena Fail




    We use Kappa and Lambda from DAKO on the Dako autostainer with Envision +.

Kappa with proteinase K,

Lambda 1:1500 with no antigen retrieval

I am at home and do not recall the dilution for Kappa. The real key to getting consistent staining with no background for these abs particularly Kappa was the use of a protein block.


Rena Fail



-----Original Message-----
From: Cheryl Powell [mailto:cherylhisto@hotmail.com]
Saturday, December 07, 2002 12:42 PM
To: histonet@pathology.swmed.edu
Subject: Kappa/Lambda IHC


Hi, Botsford recently started up an immuno program and we're constantly adding antibodies.  Kappa/Lamda has been a real pain.  We've tried several companies and so many varations it makes my head spin- but nothing is consistant.  What companies, dilutions, and antigen retrievals are any of you using?  Do you have alot of background or nonspecific staining?

Cheryl Ann Powell B.S., HTL(ASCP)

Botsford General Hospital

Farmington Hills, MI USA

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