From: | nina leek |
Adrian Leek.
Dr. Ian Montgomery wrote:
Preparing fish larvae (sea) for EM, any suggestions for a suitable buffering system given that the levels of salt in sea fish are ~3 times that of mammals. Do I simply make up the fixatives using sea water? Sorenson buffered fixatives have a high osmolarity that might be another buffer. Time, unfortunately, is short so I can't run a series of trials although I would prefer that. Back to mammals, lymphocytes and macrophage in mice, any suggestions. I was going to look for esterases as a marker for macrophage but I'm sure there is something better.
Ian.Dr. Ian Montgomery,
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biological & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 332 8855 Extn.6644.
e-mail: ian.montgomery@bio.gla.ac.uk