Judy,

If I were doing this I think I would divide the lung sucrose protect and
freeze some, paraffin embed some and keep the blocks. If you really want to
keep some in solution I think I would use 70% ETOH.

Interested in knowing others opinions as well.

Cynthia Favara
Rocky Mountain Laboratories
903 S. 4th Street
Hamilton, MT 59840
406-363-9317
FAX 406-363-9286


-----Original Message-----
From: Judy Trogadis [mailto:trogadisj@smh.toronto.on.ca]
Sent: Sunday, December 09, 2001 8:25 AM
To: histonet@pathology.swmed.edu
Subject: tissue preservation


Hello, Histonetters:

I have whole lung tissue, from rat, obtained by perfusion with 4%
paraformaldehyde, removed, and then further fixed in PFA [that's
paraformaldehyde ;-) ]  for 3 days. The plan is to collect thick vibratome
sections, so the tissue will never be embedded. I would like to keep part of
the lung for sectioning in the future and to carry out immunocytochemistry.
What solution should I keep them immersed in? Right now they are in PBS, at
4deg C  but I feel that some preservative should be added to prevent
decomposition. However, keeping them in fixative may reduce their
antigenicity.

I would appreciate hearing any suggestions from this very knowledgable
group.
Thank you
Judy

Judy Trogadis
Bio-Imaging Coordinator
St. Michael's Hospital, 8Q
30 Bond St.
Toronto, ON M5B 1W8
Canada

ph:  416-864-6060  x6337
fax: 416-864-6043
trogadisj@smh.toronto.on.ca