RE: Fixation buffer.

From:"Monson, Frederick C."

Morning (here!) Ian,
	I have been looking as well, and haven't found anything to respond
to your question, but, as usual, I have found a goldmine website for
seawater data, so I thought I would share it for those who might find a use
for artificial seawater compositions:  Another with a
PDF-publication is:

Hope it's not too late.

Fred Monson

Frederick C. Monson, PhD 
Center for Advanced Scientific Imaging(CASI) 
West Chester University of Pennsylvania 
Schmucker Science Center II  
South Church Street                                                    
West Chester, PA, 19383

> ----------
> From: 	Dr. Ian Montgomery
> Sent: 	Monday, December 3, 2001 12:01 PM
> To:
> Subject: 	Fixation buffer.
>         Preparing fish larvae (sea) for EM, any suggestions for a suitable
> buffering system given that the levels of salt in sea fish are  ~3 times
> that of mammals. Do I simply make up the fixatives using sea water?
> Sorenson buffered fixatives have a high osmolarity that might be another
> buffer. Time, unfortunately, is short so I can't run a series of trials
> although I would prefer that. 
>         Back to mammals, lymphocytes and macrophage in mice, any
> suggestions. I was going to look for esterases as a marker for macrophage
> but I'm sure there is something better.
> Ian. 
> Dr. Ian Montgomery,
> Microscopy Service Unit,
> Graham Kerr Building,
> Institute of Biological & Life Sciences,
> University of Glasgow,
> Glasgow,
> G12 8QQ.
> Tel: 0141 332 8855 Extn.6644.
> e-mail:

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