RE: Fixation buffer.
|From:||"Monson, Frederick C." |
Morning (here!) Ian,
I have been looking as well, and haven't found anything to respond
to your question, but, as usual, I have found a goldmine website for
seawater data, so I thought I would share it for those who might find a use
for artificial seawater compositions:
http://hermes.mbl.edu/BiologicalBulletin/index.html. Another with a
Hope it's not too late.
Frederick C. Monson, PhD
Center for Advanced Scientific Imaging(CASI)
West Chester University of Pennsylvania
Schmucker Science Center II
South Church Street
West Chester, PA, 19383
> From: Dr. Ian Montgomery
> Sent: Monday, December 3, 2001 12:01 PM
> To: email@example.com
> Subject: Fixation buffer.
> Preparing fish larvae (sea) for EM, any suggestions for a suitable
> buffering system given that the levels of salt in sea fish are ~3 times
> that of mammals. Do I simply make up the fixatives using sea water?
> Sorenson buffered fixatives have a high osmolarity that might be another
> buffer. Time, unfortunately, is short so I can't run a series of trials
> although I would prefer that.
> Back to mammals, lymphocytes and macrophage in mice, any
> suggestions. I was going to look for esterases as a marker for macrophage
> but I'm sure there is something better.
> Dr. Ian Montgomery,
> Microscopy Service Unit,
> Graham Kerr Building,
> Institute of Biological & Life Sciences,
> University of Glasgow,
> G12 8QQ.
> Tel: 0141 332 8855 Extn.6644.
> e-mail: firstname.lastname@example.org
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