Mary,

I have no experience with your technique. I think you are doing some sort of
InSitu?
I have had signals masked in IHC when counterstaining with Harris and
sometimes will even leave off the counterstain depending on what we are
looking for. I have had success with decolorizing hematoxylin with a dilute
<1%HCL in 70%ETOH provided the markers will with stand that.

Good luck and enjoy your Friday!
Cynthia Favara

-----Original Message-----
From: Georger, Mary [mailto:Mary_Georger@URMC.Rochester.edu]
Sent: Friday, December 07, 2001 6:52 AM
To: 'Histonet'
Subject: RISH In Situ counterstain


Good Morning,
Has anyone had experience with counterstaining RISH slides with Harris
hematoxylin rather than Mayers? A long sad story but now I find that I have
masked signal and am wondering if it could be the Harris.  Any wonderful
suggestions? I am wondering if I can take the coverslips off without
compromising the emulsion and destain in dilute HCl.
Thank  you in advance for any pearls of wisdom! 

Mary Georger
Center for Cardiovascular Research
University of Rochester Medical Center
KMRB Room 2-9816
601 Elmwood Avenue.
PO Box 679
Rochester, New York  14642
585-273-1548