advice on sectioning

From:Vanessa Heim <>

I am actually pretty new to histology and would really appreciate some
advice if possible.  I am having trouble keeping the tissues from
falling off the slides during the staining matter what
protocol I use...we use precoated slides from surgipath, and I have even
added addtional adhesive to a water bath to see if that would help the did not.  The tissues being sectioned are very fresh as
we use a cryostat to section...If anyone has any new ideas or
suggestions I would really appreciate them

Thank you

Vanessa Heim
Boston University Medical Center
Dept of Urology Research
Boston, Ma  02130

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