Re: Gurr buffer recipe?
It's pretty easy to make up from first principles and is properly referred
to as Sorensen's Phosphate Buffer. Most good Haematology text books have
recipes in their appendices, and you have a choice of molalities etc. It is
common to make up a stock 0.66Mol stock solutions and then dilute this as
high as 1:40. If you use a preservative it is an extremely stable buffer
(Is used for calibrating pH Meters and Blood Gas Machines).Roughly equal
volumes will give you a pH of 6.8-6.9, which is recommended for May Grunwald
and Jenner Giemsa and Leishman Sequences, while 6.6 is often used for
Wright's and for Rapid Diff formulations including Fields Stains A & B, pH
7.2 is recommended for peripheral blood films both thick and thin for
screening for malarial parasites (Easier to see Chromatin dot).
The only reason a lot of people used to use the Gurr product was that it was
in compressed tablet form, with one tablet making 500mls of buffer, the
tablets were extremely stable and a shelf life of ten or so years could be
expected if you kept it tightly closed.
Regards Mike Rentsch
Australian Biostain (Downunder)
From: Tamara Howard <firstname.lastname@example.org>
To: Histology listserver <email@example.com>; Microscopy
Date: Thursday, 7 December 2000 9:04
Subject: Gurr buffer recipe?
>Would anyone happen to have the actual recipe for Gurr's buffer? I think
>it is some phosphate formulation - we've been hunting the recipe and have
>found some refs, but will have to interlibrary the journals/books and we
>thought we'd give the 'net a try, first.
>I know we can buy the pre-made buffer, but we need such a tiny amount that
>we'd rather make it from scratch (plus we want to know what it is!).
>Cold Spring Harbor Laboratory, NY
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