HI Kim,
   For GMS try using a 4% chromic acid for an hour at room temp or a 0.5%
periodic acid for 15 min followed by a ten min wash--faster and safer!

Cindy Tily,HTL
Coll of Vet Med
Auburn Univ
----- Original Message -----
From: <Kimcatk@aol.com>
To: <histonet@pathology.swmed.edu>
Sent: Thursday, December 07, 2000 1:47 PM
Subject: GMS & Steiner Chapman / Re: Seeking Staining Procedures


> Dear HistoNet colleagues,
>      It is great to have HistoNet working again!  Thank you to those of
you who responded to my posting.  I got some leads on staining for fungi and
spirochetes, but I still have some questions.
>      Has anyone done Grocott's Methenamine Silver (GMS) without heating
the chromic acid?  I heard that it is possible to oxidize in the chromic
acid for an hour instead of heating it, but I do not have a procedure.
>      Also, I am looking for a procedure for Steiner Chapman because that
replaces the uranium/uranyl nitrate with some type of zinc solution.
>      I would be very grateful for any help you could provide.
>
> Sincerely,
>
> Kimberly Atkin HT (ASCP)
> Boston, MA
>
>
> In a message dated Tue, 5 Dec 2000  8:52:00 AM Eastern Standard Time,
Kimcatk writes:
>
> << Dear HistoNet colleagues,
>
>      I am seeking to replace some staining procedures in my laboratory
with NON-microwave methods.  Optimally, I am hoping to find stains that are
free of heavy metals or any particularly toxic or dangerous components.  I
can not use mercury.
>
>      I need to find staining protocols or kits to demonstrate the
following:
>
> Fungal organisms
> Spirochetes, Campylobacter, and Legionella organisms
>
>      In addition, the gram stain procedure I use is very cumbersome and I
would be extremely grateful for a simpler method.
>
>      Thank you all very much in advance for your help.
>
> Sincerely,
>
> Kimberly Atkin HT (ASCP)
>
> Boston, Massachusetts
>
>  >>
>
>
>