Hi

I need to visualise CD31 in rat tissue. There appears to be only one
monoclonal available from Research Diagnostics. It requires zinc fixation
prior to paraffin embedding and  alk. phos detection.

Does anyone have an opinion on
1. Zn fixation
2.what is the optimal chromogenic substrate for alk phos
3. Dako mentions an endogenous alk phos inhibitor, levamisole which they
put in some of their kits

Thanks


_____________________________________________________

Nazlia Samodien

Cardiovascular Research Unit
Dept. of Cardiothoracic Surgery
University of Cape Town Medical School
Anzio Rd, Observatory, 7925, South Africa

Tel. +27 21 406 6398 (office)
        +27 21 406 6476 (Secretary)
Fax + 27 21 448 5935


Email: ctssamodien@samiot.uct.ac.za