Hi Kim,

Simply oxidize the sections in 5% chromic acid for an hour at room
temperature,
wash in running water for 3-5 minutes,
treat with 1% sodium bisulfite for a minute (this removes any residual
chromic acid),
wash in running water for 5 minutes,
rinse in distilled water
proceed staining.....

Jim

____________________________
James E. Staruk, HT(ASCP)
Mass Histology Service
http://www.masshistology.com

> -----Original Message-----
> From: Kimcatk@aol.com [mailto:Kimcatk@aol.com]
> Sent: Thursday, December 07, 2000 1:47 PM
> To: histonet@pathology.swmed.edu
> Subject: GMS & Steiner Chapman / Re: Seeking Staining Procedures
>
>
> Dear HistoNet colleagues,
>      Has anyone done Grocott's Methenamine Silver (GMS) without
> heating the chromic acid?  I heard that it is possible to oxidize
> in the chromic acid for an hour instead of heating it, but I do
> not have a procedure.
>
> Sincerely,
>
> Kimberly Atkin HT (ASCP)
> Boston, MA