RE: blood inside blood vessels

You can always lyse red blood cells with methanol, it won't get rid of them
but may keep them from interfering.

		-----Original Message-----
		From:	Circle :> []
		Sent:	Friday, December 01, 2000 5:58 PM
		Subject:	blood inside blood vessels

		Dear histoners,

		I cannot do any perfusion of the blood vessels samples as I
need an intact
		endothelium for other kind of experiments. So, I only
isolated the blood
		vessels and froze it in liquid nitrogen. 

		Yesterday, I did frozen section and found that there were
much blood
		inside the lumen. Two questions comes up:

		1. Is it possible to get rid of the blood cells?
		2. Is the preservation method (by freezing the samples in
		nitrogen) optimal to preservation the antigens on the
endothelial cells so
		I can use IHC to stain the endothelial cells?

		Thank you very much!



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