RE: blood inside blood vessels
You can always lyse red blood cells with methanol, it won't get rid of them
but may keep them from interfering.
From: Circle :> [mailto:firstname.lastname@example.org]
Sent: Friday, December 01, 2000 5:58 PM
Subject: blood inside blood vessels
I cannot do any perfusion of the blood vessels samples as I
need an intact
endothelium for other kind of experiments. So, I only
isolated the blood
vessels and froze it in liquid nitrogen.
Yesterday, I did frozen section and found that there were
inside the lumen. Two questions comes up:
1. Is it possible to get rid of the blood cells?
2. Is the preservation method (by freezing the samples in
nitrogen) optimal to preservation the antigens on the
endothelial cells so
I can use IHC to stain the endothelial cells?
Thank you very much!
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