In-situ microtomy

From:"Patrick M. Haley " <>

Fellow Netters,
    I have  a researcher wanting to perform in-situ on mouse feet. They are
looking at an arthritis model. The specimens will be fixed in
Methyl-Carnoy's. I have two questions:

Can these be decalcified without loosing DNA/RNA, and what is the reagent of
If they can not be decalcified, what is the best method of microtomy  to
produce usable sections?

Many Thanks!!!

Pat Haley
HTN, inc.

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