Fellow Netters,
    I have  a researcher wanting to perform in-situ on mouse feet. They are
looking at an arthritis model. The specimens will be fixed in
Methyl-Carnoy's. I have two questions:

Can these be decalcified without loosing DNA/RNA, and what is the reagent of
choice?
If they can not be decalcified, what is the best method of microtomy  to
produce usable sections?

Many Thanks!!!

Pat Haley
HTN, inc.
www.histology.net