For those antibodies not offered by Ventana we purchase from another vendor
and put into Ventana dispensers.

Jennifer
San Antonio Community Hospital

> ----------
> From: 	Patricia Karlisch[SMTP:PKARLISCH@PSGHS.EDU]
> Sent: 	Friday, August 13, 1999 10:52 AM
> To: 	Histonet@pathology.swmed.edu
> Subject: 	Automatic Staining on IHC
> 
> Dear Histonetters:
>  For those of you who do IHC staining using the Ventana ( a great
> instrument) what do you do for those antibodies that are not offered by
> Ventana for automatic staining.  Do you proceed manually or do you use a
> different autostainer?  Are you using kits or making the antibody
> dilutions
> yourself?  How does this affect your workflow?   The autostainer enhances
> the workflow tremendously but the outliers are labor intensive and I am
> just
> curious how most people handle this .  We do about 80-150 slides a day
> using
> 14-20 different antibodies ( of about 110 different antibodies). About
> 50-70% are done on the Ventana daily depending on what is being ordered.  
> Thank you
> 
> Pat Karlisch
> Geisinger Medical Center
> Danville, Pa  17822
> pkarlisch@psghs.edu
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