LR White

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From:Sarka Lhotak <lhotaks@fhs.csu.McMaster.CA>
To:histonet@pathology.swmed.edu
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Date:Wed, 18 Aug 1999 10:48:11 -0400 (EDT)
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Hi Jill,
	I used to embed piglet bones into LR White. I used plastic molds
(Polyscience Inc. Cat# 18646B) which I covered/sealed with SaranWrap to
prevent oxygen exposure. I polymerized the resin by UV light at -20C.
Bubbles of soft unpolymerized resin were sometimes a problem. I cut
sections on Jung microtome that is used for cutting non-decalcified bones.
I don't think you will be able to cut LR White with disposable blades. I
experimented also with Spurr's resin and found its polymerization to be
more reliable. If you need more detail, please, contact me,

Sarka Lhotak

Hamilton Regional Cancer Centre
McMaster University
Hamilton, Ontario, Canada

lhotaks@mcmaster.ca




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